CAIE Biology (9700)

The wall of the small intestine is highly folded to form villi. Between the v illi are infoldings known Fig. 1.1 is a diagram of a section through a single villus and a crypt of Lieberkühn. lacteal capillary network enterocytegut lumen goblet cell enteroendocrine cell Paneth cell venule lymph vessel intestinal stem cell crypt of Lieberkühn Fig. 1.1 shows that the epithelium of the villus contains mainly goblet cells and cells known as enterocytes. Both cell types have microvilli on the apical surface (surface facing the gut lumen). * 0000800000002 * 9TTTTuTTTTTTTi9 Goblet cells are involved in the production of mucus. Enterocytes are adapted for the absorption of the soluble products of digestion. These products enter the circulatory system.

A student incorrectly stated that an enterocyte has many cilia on its apical surface. Explain the dif ference between a cilium and a microvillus.

Suggest one role of the mucus produced by the goblet cells of the villus.

In Fig. 1.1, blood vessel X delivers blood to the capillary network of the villus, where tissue fluid is formed. Some of the fluid passes back into the capillaries and then into the venule.

Blood vessel X receives blood from an artery . Name the type of blood vessel represented by X.

Describe the formation of tissue fluid in the capillary network of the villus. 9TTTTuTTTTTTTs9

In response to the presence of compounds in the gut lumen, enteroendocrine cells synthesise

Paneth cells, which are secretory cells, are located between intestinal stem cells at the base of the crypt of Lieberkühn, as shown in Fig. 1.1. A Paneth cell has a very different appearance to an intestinal stem cell. Fig. 1.2 is a transmission electron micrograph of a Paneth cell. Paneth cell nucleus nucleus of adjacent intestinal stem cell Fig. 1.2 * 0000800000004 * 9TTTTuTTTTTTT 9

Paneth cells are formed following the mitotic division of an intestinal stem cell during a cell cycle. Complete the cell cycle shown in Fig. 1.3 by naming, in sequence, the stages of mitosis. interphase mitosis names of stages of mitosis cytokinesis

One of the functions of a Paneth cell is to synthesise and secrete peptides and proteins that act against pathogens in the gut lumen. State and explain the evidence, visible in Fig. 1.2, which suggests that a Paneth cell:

Explain why a Paneth cell has a very different appearance to an intestinal stem cell. [Total: 14] * 0000800000005 * 9TTTTuTTTTTTTd9

Cholera is an infectious intestinal disease caused by a bacterial pathogen.

Name the species of bacterium that causes cholera.

The W orld Health Organization (WHO) recommends a number of dif ferent approaches for the prevention and control of cholera. Two of these are: Suggest and explain how these two approa ches help in the prevention and control of cholera. 9TTTTuTTTTTTTo9

Mass vaccination using an oral cholera vaccine (OCV) can be carried out in situations where there is a high risk of people developing the disease. Passive immunisation involves transferring antibodies into a person for the prevention or treatment of an infectious disease. Some of the infectious diseases for which passive immunisation is available use monoclonal antibody . Passive immunisation for cholera using monoclonal antibody could be available in the future.

Complete Table 2.1 to compare an OCV and passive immunisation for cholera: Table 2.1 row feature oral cholera vaccine passive immunisation for cholera component causing the desired response antibody 2 type of immunity gained artificial active artificial passive stimulates production of memory lymphocytes yes / no yes / no length of time needed to have an effect shorter same longer longer same shorter

duration of immunity shorter same longer longer same shorter

The production of monoclonal antibodies for treatment involves the form ation of hybridoma cells from two different cell types. Name the two types of cell that fuse to form a hybridoma cell and explain why this fusion is necessary. [Total: 12] * 0000800000007 * 9TTTTuTTTTTTTc9 3 The gene LCT codes for the enzyme lactase. In babies, lactase synthesis is necessary for digesting lactose, the sugar found in milk. Another gene, MCM 6, has introns that have a regulatory role in the expression of gene LCT. Gene MCM 6 codes for a protein that has no involvement in lactase synthesis. As children get older , the MCM 6 introns are responsible for a decrease in lactase synthesis.

LCT and MCM 6 are located on the same chromosome in humans. Suggest dif ferences between gene LCT and gene MCM 6, other than their locations in different positions on the same chromosome.

With reference to the process of lactase synthesis, explain the relationship between:

A mutation in a regulatory intron of MCM 6 allows lactase synthesis to continue. This is known as lactase persistence. In this mutation, the number of nucleotides in the intron remains the same, but one of the nucleotides is different to the original nucleotide. State the type of mutation that is the cause of lactase persistence. 9TTTTuTTTTTTTu9

Fig. 3.1 summarises the reaction catalysed by lactase. lactose galactose α-glucose lactase O OH symptoms, such as abdominal pain, if their diet contains lactose. consumed to avoid symptoms of lactose intolerance. A student compared the activity of two dif ferent concentrations of a lactase supplement using an artificial substrate, ONPG, instead of lactose. A solution of ONPG is colourless, but the hydrolysis of ONPG by lactase releases a coloured product. The student planned to follow the progress of the reaction for each concentration of lactase using a colorimeter . Explain why the student chose to use a colorimeter to follow the progress of the reaction for each concentration of lactase. 9TTTTuTTTTTTTm9

The lactose in milk can be hydrolysed using immobilised lactase or using lactase free in solution (free lactase). This results in milk and milk products that do not contain lactose Scientists carried out an investigation to compare the activity of lactase immobilised in very small magnetic beads (magnetic microspheres) with free lactase, at dif ferent temperatures and at different pH values. Fig. 3.2 shows the activity of immobilised lactase and the activity of free lactase at 5 different temperatures. Fig. 3.3 shows the activity of immobilised lactase and the activity of free lactase at 8 different pH values. 35 40 45 temperature / °C percentage of maximum activity 50 55Key = immobilised lactase = free lactase 5.5 6.5 7.5 percentage of maximum activity

56.0 7.0 8.0 9.0Key = immobilised lactase = free lactase Fig. 3.3 * 0000800000010 * 9TTTTuTTTTTThT9 One advantage of using magnetic microspheres with immobilised lactase is that they can be easily recovered using an electric field and reused. With reference to Fig. 3.2 and Fig. 3.3, explain why the results indicate that there are other advantages in using immobilised lactase instead of free lactase to produce [Total: 13] * 0000800000011 * 9TTTTuTTTTTThh9 4 Bacteria can be classified according to the type of cell wall they have. external environment cytoplasm lipopolysaccharide outer membrane protein periplasmic proteins peptidoglycan layer outer membrane cell wall periplasm cell surface membrane

With reference to Fig. 4.1, outline the similarities and dif ferences between the outer membrane 9TTTTuTTTTTThi9 contain ATP and DNA. periplasmic proteins different cell productsKey O-IMV plasmids (small, circular DNA) cell surface membrane outer membrane of cell wall peptidoglycan layerOMV molecules in outer membrane and cell surface membrane antibiotic resistance. [Total: 7] * 0000800000013 * 9TTTTuTTTTTThs9 5 A plant that is described as a mesophyte has ev olved to grow in conditions that do not normally experience water stress (low availability of water).

Fig. 5.1 is a diagram of a cross section through the leaf of a herbaceous dicotyledonous mesophyte. Complete Fig. 5.1 by naming structure A and tissue layer B. upper epidermis palisade mesophyll vein intercellular air space lower epidermis

In xerophytes, some of the structural features shown in Fig. 5.1 are modified as adaptations for surviving conditions of water stress. Complete Table 5.1 to show how the structural feature listed may be modified in the leaf of a xerophyte. Each feature should have a different example of a modification. Table 5.1 structural feature in Fig. 5.1one example of a xerophytic adaptation structure A upper epidermis lower epidermis [3] [Total: 5] * 0000800000014 * 9TTTTuTTTTTTh 9 6 In the pulmonary circulation of a mam mal, deoxygenated blood becomes oxygenated when red blood cells pass through alveolar capillaries and haemoglobin within the cells combines with oxygen. The blood returns to the heart to be pumped around the systemic circulation.

Describe the sequence of events occurring in the heart that allows blood returning in the pulmonary circulation to then enter the systemic circulation. Y ou should include in your description: circulation that are connected to the heart Y ou do not need to include details of control of the cardiac cycle. 9TTTTuTTTTTThd9

Fig. 6.1 is a drawing of a haemoglobin molecule to show its globular structure.

Describe the quaternary structure of a haemoglobin molecule.

Outline how a haemoglobin molecule can become fully saturated with oxygen to form oxyhaemoglobin. [Total: 9] The boundaries and names shown, the designations used and the presentation of material on any maps contained in this question paper/insert do not imply official endorsement or acceptance by Cambridge Assessment International Education concerning the legal status of any country , territory, or area or any of its authorities, or of the delimitation of its frontiers or boundaries. reasonable ef fort has been made by the publisher (UCLES) to trace copyright holders, but if any items requiring clearance have unwittingly been included, the publisher will be pleased to make amends at the earliest possible opportunity. Assessment International Education Copyright Acknowledgements Booklet. This is produced for each series of examinations and is freely available to download at www.cambridgeinternational.org after the live examination series. Local Examinations Syndicate (UCLES), which is a department of the University of Cambridge. * 0000800000016 * 9TTTTuTTTTTTho9